|
KMID : 0385520140270030140
|
|
Analytical Science & Technology
2014 Volume.27 No. 3 p.140 ~ p.146
|
|
|
Study of improving precision and accuracy by using an internal standard in post column isotope dilution method for HPLC-ICP/MS
|
|
Joo Min-gyu
Park Myung-Sun
Pak Yong-Nam
|
|
|
Abstract
|
|
|
|
An internal standard was used in PCID (post column isotope dilution) to improve the accuracy and precision in quantification of various chemical species. The error occurring in the column was the largest in HPLC-ICP/MS (high performance liquid chromatography-inductively coupled plasma/mass spectrometry) when PCID and other traditional quantification methods were compared with each other. Internal standard was effective in correcting the loss of sample in the column to improve accuracy and precision. When applied to SeMet, using MeSecys or Se4+as an internal standard, relative errors were reduced from 31% and 13% to less than 1%, while standard deviations were reduced from 5.1% and 6.9% to 1.5% and 0.2%, respectively. Positive aspects of using an internal standard in PCID were compared with other quantitative techniques and discussed in detail.
|
|
|
KEYWORD
|
|
|
internal standard, post column isotope dilution, species unspecific ID, HPLC-ICP/MS,
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|